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mouse anti-src-2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher mouse anti-src-2
    Mouse Anti Src 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-src-2+mouse/mouse+anti+src+2/pm31997259-91-38-40
    Average 90 stars, based on 1 article reviews
    mouse anti-src-2 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Interaction of steroid receptor coactivators and estrogen receptors in the human placenta
    Article Snippet: After blocking, membranes 166 were incubated with anti-ERα rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-ERβ 167 rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-SRC-1 mouse (Thermo Fisher 168 Scientific, Waltham, MA, diluted 1:500), anti-SRC-2 mouse (Thermo Fisher Scientific, 169 diluted 1:1000), and anti-SRC-3 mouse antibodies (Santa Cruz Biotechnology, diluted 1:1000) 170 for overnight, followed by HRP-conjugated anti-rabbit and anti-mouse secondary antibodies 171 (diluted 1:2000) in 5% skim milk with PBS-T for 1 h. Luminol reagent (Bio-Rad, Hercules, 172 CA, USA) was used to visualize antibody binding.

    Incubation:

    Article Title: Interaction of steroid receptor coactivators and estrogen receptors in the human placenta
    Article Snippet: After blocking, membranes 166 were incubated with anti-ERα rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-ERβ 167 rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-SRC-1 mouse (Thermo Fisher 168 Scientific, Waltham, MA, diluted 1:500), anti-SRC-2 mouse (Thermo Fisher Scientific, 169 diluted 1:1000), and anti-SRC-3 mouse antibodies (Santa Cruz Biotechnology, diluted 1:1000) 170 for overnight, followed by HRP-conjugated anti-rabbit and anti-mouse secondary antibodies 171 (diluted 1:2000) in 5% skim milk with PBS-T for 1 h. Luminol reagent (Bio-Rad, Hercules, 172 CA, USA) was used to visualize antibody binding.

    Binding Assay:

    Article Title: Interaction of steroid receptor coactivators and estrogen receptors in the human placenta
    Article Snippet: After blocking, membranes 166 were incubated with anti-ERα rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-ERβ 167 rabbit (Santa Cruz Biotechnology, diluted 1:500), anti-SRC-1 mouse (Thermo Fisher 168 Scientific, Waltham, MA, diluted 1:500), anti-SRC-2 mouse (Thermo Fisher Scientific, 169 diluted 1:1000), and anti-SRC-3 mouse antibodies (Santa Cruz Biotechnology, diluted 1:1000) 170 for overnight, followed by HRP-conjugated anti-rabbit and anti-mouse secondary antibodies 171 (diluted 1:2000) in 5% skim milk with PBS-T for 1 h. Luminol reagent (Bio-Rad, Hercules, 172 CA, USA) was used to visualize antibody binding.



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    Image Search Results


    (A) 3C assays identify various pairs of contacts within the GREB1 gene in hormone-deprived MCF-7 cells (0′) and after 60 minutes of 100 nM E2 treatment (60′). The Looping Index3C was normalized to the ligated PstI-cut BAC clone CTD-2563K11. The values for each interaction pair in untreated (0′) cells were set to 1, resulting in “Fold-change in Looping Index3C”. Two gene-body SRC-3 binding sites, GBS1 and GBS2, were assayed as indicated. Interrogated pairs of contacts are shown. E, Pr, Po, G1 and G2 represent Enhancer, preTSS promoter, postTSS promoter, GBS1 and GBS2, respectively. Location of control sequence F2 is indicated. Grey arrow near G1 (GBS1) indicates a control sequence (GBS1up). See also Figure S1L.

    Journal: Molecular cell

    Article Title: SRC-3 coactivator governs dynamic estrogen-induced chromatin looping interactions during transcription

    doi: 10.1016/j.molcel.2018.04.014

    Figure Lengend Snippet: (A) 3C assays identify various pairs of contacts within the GREB1 gene in hormone-deprived MCF-7 cells (0′) and after 60 minutes of 100 nM E2 treatment (60′). The Looping Index3C was normalized to the ligated PstI-cut BAC clone CTD-2563K11. The values for each interaction pair in untreated (0′) cells were set to 1, resulting in “Fold-change in Looping Index3C”. Two gene-body SRC-3 binding sites, GBS1 and GBS2, were assayed as indicated. Interrogated pairs of contacts are shown. E, Pr, Po, G1 and G2 represent Enhancer, preTSS promoter, postTSS promoter, GBS1 and GBS2, respectively. Location of control sequence F2 is indicated. Grey arrow near G1 (GBS1) indicates a control sequence (GBS1up). See also Figure S1L.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SMC1 Bethyl Laboratories A300-055A SMC3 Bethyl Laboratories A300-060A RAD21 Bethyl Laboratories ab154769 MED12 Bethyl Laboratories A300-774A CDK8 Bethyl Laboratories A302-500A HDAC1 Bethyl Laboratories A300-713A SRC-1 Santa Cruz Biotechnology 1135/H4; sc-32789 SRC-2 BD Biosciences 610985 SRC-3 Custom mouse mAb generated at Baylor College of Medicine N/A RNA Pol II (RPB1) Active Motif 101307 ERα Santa Cruz Biotechnology HC-20; sc-543 YY1 Bethyl Laboratories A302-779A Mouse IgG Santa Cruz Biotechnology sc-2025 Rabbit IgG Santa Cruz Biotechnology sc-2027 ECL Anti-Rabbit IgG-HRP conjugate GE Healthcare UK NA934V ECL Anti-Mouse IgG-HRP conjugate GE Healthcare UK NA931V Chemicals, Peptides, and Recombinant Proteins TRI-Reagent Molecular Research Center TR-118 BAN (4-bromoanisole) Molecular Research Center BN-191 Triptolide Sigma-Aldrich T3652 Flavopiridol Sigma-Aldrich F3055 Poly(vinyl alcohol) Sigma-Aldrich P8136 Q5 High-Fidelity DNA Polymerase New England Biolabs M0491 OneTaq Hot Start DNA Polymerase New England Biolabs M0481L Pst I New England Biolabs R0140L Recombinant estrogen receptor alpha (ERα) Thermo Fisher Scientific A15674 T4 DNA Ligase New England Biolabs M0202S 17-β Estradiol (E2) (water-soluble) Sigma-Aldrich E4389 Glycogen Sigma-Aldrich G1767 Critical Commercial Assays TURBO DNA-free kit Invitrogen/Ambion AM1907 SensiFAST SYBR Hi-ROX One-Step kit Bioline BIO-73005 SYBR Green PCR Master Mix Applied Biosystems 4309155 Gibson Assembly Cloning Kit New England Biolabs E5510S TOPO TA Cloning Kit Invitrogen K450001 Lipofectamine RNAiMax Invitrogen 13778150 ECL Plus Western Blotting Substrate Pierce 32132 Experimental Models: Cell Lines MCF-7 Tissue Culture Core, Baylor College of Medicine N/A HeLa Tissue Culture Core, Baylor College of Medicine N/A HeLa S3 (for making bulk NE) Cell Culture Company, LLC HA48 Oligonucleotides Please see Supplemental Table S1 Recombinant DNA: GREB1 BAC clone CTD-3138J7 Invitrogen CTD-3138J7 GREB1 BAC clone CTD-2563K11 Invitrogen CTD-2563K11 NRIP1 BAC clone RP11-1025G18 Invitrogen RP11-1025G18 FOS BAC clone CTD-2655F5 Invitrogen CTD-2655F5 pIE-0 ( Pazin et al., 1994 ) NA Other M280 Streptavidin Dynabeads™ Invitrogen 60210 Protein A Agarose Invitrogen 15918-014 Protein G Sepharose 4B Invitrogen 101242 Quantum Prep PCR Kleen Spin Columns Bio-Rad 732-6300 Open in a separate window KEY RESOURCES TABLE

    Techniques: Binding Assay, Sequencing